Aktivitas Analgesik Ekstrak Etanol Daun Pucuk Merah (Syzygium myrtifolium Walp.) pada Mencit Putih Jantan (Mus musculus)
Abstract
Analgesics are substances that reduce or eliminate pain without causing loss of consciousness. Traditional medicines are considered safer than synthetic drugs. One plant with potential as an analgesic is the red shoot plant (Syzygium myrtifolium Walp). This plant contains flavonoids, anthocyanins, phenolics, and triterpenoids. This study aimed to determine the analgesic activity of ethanol extract of red shoot leaves (EPM) in male white mice. The analgesic effect was obtained through the writhing test using 1% glacial acetic acid induction. Twenty male white mice were divided into 5 treatment groups, including negative control (0.5% Na CMC), treatment 1 (EPM 3.7 mg/kgBW), treatment 2 (EPM 7.4 mg/kgBW), and treatment 3 (EPM 14.8 mg/kgBW), and positive control (mefenamic acid 500 mg/70 kgBW). All mice were given treatment according to their respective groups. After 30 minutes, all mice were induced with 1% glacial acetic acid at a dose of 10 ml/kgBW intraperitoneally. Then, the number of writhes was observed every 5 minutes for 60 minutes. The average number of writhes data was analyzed using ANOVA and Tukey HSD tests. The results showed that treatment groups 2 (7.40 mg/kgBW) and 3 (14.80 mg/kgBW) showed analgesic activity in male white mice induced by 1% acetic acid. The best dose was 14.80 mg/kgBW with writhing protection of 34.74% and analgesic efficacy of 45.59% compared to mefenamic acid.
Downloads
References
A. A. . Tamimi, E. De Queljoe, and J. P. Siampa, “UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KELOR (Moringa oleifera Lam.) PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus),” Pharmacon, vol. 9, no. 3, p. 325, 2020, doi: 10.35799/pha.9.2020.30015.
P. V. Pandey, W. Bodhi, and A. Yudistira, “Uji Efek Analgetik Ekstrak Rumput Teki (Cyperus Rotundus L.) Pada Tikus Putih Jantan Galur Wistar (Rattus Novergicus),” vol. 2, no. 02, p. 6, 2013, [Online]. Available: http://files/167/Pandey et al. - 2013 - UJI EFEK ANALGETIK EKSTRAK RUMPUT TEKI (CYPERUS RO.pdf
L. Indriani et al., “Potensi Aktivitas Ekstrak Etanol Daun Pucuk Merah (Syzygium myrtifolium Walp.) sebagai Antihiperurisemia pada Mencit Putih ( Mus musculus),” J. Pharm. Umr., vol. 1, no. 1, pp. 1–10, 2023, [Online]. Available: https://ejurnal.umri.ac.id/index.php/JFMu
E. D. Firdaus, “Cuka, Efektivitas Bawang, Apel Terhadap, Sebagai Analgesik Yang, Mencit Asam, Diinduksi,” Skripsi. Univ. Jember, 2019.
Nursyafni, A. Rahmawati, L. Indriani, and D. H. Ashari, “Anti-inflammatory Activity of an Ethanol Extract of Pucuk Merah ( Syzigium myrtifolium Walp .) In Vivo,” J. Sains Farm. Klin., vol. 10, no. 3, pp. 286–292, 2023, doi: 10.25077/jsfk.10.3.286-292.2023.
L. Indriani, E. Mulyati Effendi, and K. C. Fadillah, “Acute toxicity test of 96% ethanol extract of syzygium myrtifolium leaves in white mice (Mus musculus),” Pharm. Educ., vol. 21, no. 2, pp. 201–204, 2021, doi: 10.46542/pe.2021.212.201204.
L. Indriani, A. Almasyhuri, and A. R. Pratama, “Aktivitas Gel Ekstrak Etanol Daun Pucuk Merah (Syzygium myrtifolium) Terhadap Penyembuhan Luka Bakar Tikus Sprague-Dawley,” FITOFARMAKA J. Ilm. Farm., vol. 10, no. 2, pp. 178–187, 2020, doi: 10.33751/jf.v10i2.2233.
N. A. Haryati, C. Saleh, and Erwin, “Uji Toksisitas Dan Aktivitas Antibakteri Ekstrak Daun Merah Tanaman Pucuk Merah (Syzygium Myrtifolium Walp.) Terhadap Bakteri,” J. Kim. Mulawarman, vol. 13, no. 1, pp. 35–40, 2015, [Online]. Available: http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/43
Moerfiah, L. Indriani, and R. Pramayudha, “Utilizing of 96% etanol extract activity red leaf (Syzygium myrtifolium walp.) maseration and socletation method as an antidiarrhea at male mice,” Int. J. Recent Technol. Eng., vol. 8, no. 2 Special Issue 7, 2019, doi: 10.35940/ijrte.B1045.0782S719.
Depkes, Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Direktorat Jendral Pengawasan Obat dan Makanan, 2000.
S. S. Yuwono and D. R. Faustina, “Effect of withering time and chopping size on properties of pucuk merah (Syzygium oleana) herbal tea,” IOP Conf. Ser. Earth Environ. Sci., vol. 230, no. 1, 2019, doi: 10.1088/1755-1315/230/1/012047.
N. Sofiyanti, D. Iriani, and A. R. Lestari, “Kajian Anatomi-Histokimia Tangkai Daun dan Karakteristik Epidermis Pucuk Merah (Syzygium myrtifolium Walpp. – Myrtaceae),” Bul. Anat. dan Fisiol., vol. 7, no. 2, pp. 83–90, 2022, doi: 10.14710/baf.7.2.2022.83-90.
O. M. Prameswari and S. B. Widja, “The Effect of Water Extract of Pandan Wangi Leaf to Decrease Blood Glucose Levels and Pancreas Histopathology at Diabetes Mellitus Rats,” J. Pangan dan Agroindustri, vol. 2, no. 2, pp. 16–27, 2014, [Online]. Available: https://jpa.ub.ac.id/index.php/jpa/article/view/33/40
A. Widiyanto, K. A. Pradana, F. Hidayatullah, J. Tri, N. S. Putra, and A. S. Fajriah, “Nyeri Asam Urat Pada Lansia Di Desa,” J. Chem. Inf. Model., vol. 3, no. 2, pp. 103–113, 2020, [Online]. Available: https://jurnal.stikesmus.ac.id/index.php/avicenna/article/view/422
G. Gunawan, T. Chikmawati, S. Sobir, and S. Sulistijorini, “Review: Fitokimia genus Baccaurea spp.,” Bioeksperimen J. Penelit. Biol., vol. 2, no. 2, p. 96, 2016, doi: 10.23917/bioeksperimen.v2i2.2488.
F. Nola, G. K. Putri, L. H. Malik, and N. Andriani, “Isolasi Senyawa Metabolit Sekunder Steroid dan Terpenoid dari 5 Tanaman,” Syntax Idea, vol. 3, no. 7, pp. 1612–1619, 2021, doi: 10.46799/syntax-idea.v3i7.1307.
M. T. Dolorosa, Nurjanah, S. Purwaningsih, E. Anwar, and T. Hidayat, “Kandungan Senyawa Bioaktif Bubur Rumput Laut Sargassum Plagyophyllum Dan Eucheuma Cottonii Sebagai Bahan Baku Krim Pencerah Kulit,” Jphpi, vol. 20, no. 3, pp. 633–644, 2017.
T. Sentat, S. Pangestu, and A. F. Samarinda, “Uji Efek Analgesik Ekstrak Etanol Daun Kresen (Muntingia calabura L.) Pada Mencit Putih Jantan (Mus musculus) Dengan Induksi Nyeri Asam Asetat,” J. Ilm. Manuntung, vol. 2, no. 2, pp. 147–153, 2016.
Goodman and Gilman’s, Pharmacology and Therapeutics., vol. 137, no. 3520. 1891. doi: 10.1016/S0140-6736(02)18086-X.
M. L. Puspitasari, T. V. Wulansari, T. D. Widyaningsih, and J. Mahar, “AKTIVITAS ANTIOKSIDAN SUPLEMEN HERBAL DAUN SIRSAK ( Annona muricata L .) DAN KULIT MANGGIS ( Garcinia mangostana L .) : KAJIAN PUSTAKA Antioxidant Activity Herbal Supplements of Soursop Leaf ( Annona muricata L .) and Pericarp of Mangosteen ( Garcinia man,” Pangan Dan Agroindustri, vol. 4, no. 1, pp. 283–290, 2016.
Zulkifli and E. E. Octaviany, “Uji Efek Analgetik Ekstrak Akar Binasa (Plumbago indica L) Asal Kabupaten Sidenreng Rappang Terhadap Mencit Dengan Metode Writhing Reflex Test,” J. Herb. Indones., vol. 1, no. 1, pp. 43–49, 2019.
