AKTIVITAS ANTIDIABETES EKSTRAK TANGKAI DAUN TALAS PADANG (COLOCASIA GIGANTEA BLUME HOOK.F) SECARA IN VITRO MELALUI INHIBISI ENZIM Α- GLUKOSIDASE

  • Hesti Marliza Program Studi Farmasi, Institut Kesehatan Mitra Bunda , Jl. Seraya No.1 Batam
  • Sri Rahayu Melpa Hasni Hasni Program Studi Farmasi, Institut Kesehatan Mitra Bunda , Jl. Seraya No.1 Batam
  • - Suhaera Program Studi Farmasi, Institut Kesehatan Mitra Bunda , Jl. Seraya No.1 Batam
Keywords: Colocasia gigantea Hook.f, Diabetes mellitus, α-glucosidase, inhibition

Abstract

The leaf of taro Padang (Colocasia gigantea Blume Hook.f) is a traditional plant that is effective as an alternative diabetes treatment. Diabetes mellitus (DM) is a metabolic disorder of carbohydrate and fat metabolism. This study aims to determine the in vitro antidiabetic activity of taro leaf stalk extract (Colocasia gigantean Blume Hook.f) through inhibition of the α-glucosidase enzyme. The antidiabetic activity test was carried out by measuring the percent inhibition of the extract against the α-glucosidase enzyme in vitro. The activity of α-glucosidase was determined by measuring p-nitrophenol which was produced from the reaction of the enzyme and the p-nitrophenyl-α-D-glucopyranoside (p-NPG) substrate using a microplate reader at a wavelength of 410 nm. The results showed that the extract of taro leaf stalks (Colocasia gigantean Blume Hook.f) was active as an antidiabetic because it could inhibit the action of the α-glucosidase enzyme which was aimed at IC50 values ​​<100 µg / mL. ppm of 98.35% with an IC50 value of 31.5670 ppm.

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Published
2021-10-05
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