Optimization of Nitrogen and Mineral Sources in Liquid Media for Amylase Production Thermophilic Aspergillus sp. LBKURCC304

  • Mhd Muslim Syaifullah Universitas Riau
  • Silvera Devi Universitas Riau
  • Saryono Universitas Riau
  • Itnawita Universitas Riau
  • Mukhlis Universitas Riau
Keywords: Optimization, Amylase, Nitrogen, Minerals

Abstract

The application of amylase is relatively large, used in various industries: textiles, pulp and paper, feed, detergents, and food. Therefore, the isolation and optimization of amylase production from various microorganisms has been ongoing. Optimization of amylase production from Aspergillus sp. LBKURCC304 was carried out in liquid media with variable sources of nitrogen (soy flour, tempeh flour or catfish flour) and minerals (FeSO47H2O; CaCl2 2H2O; MgSO4 7H2O; MnSO4 H2O, BaCl2 2H2O) incubated at 50⁰C for 11 days with an agitation speed of 150 rpm. The amylase activity produced was determined by the Nelson-Semogyi method, the protein content by the Lowry method and the specific activity was calculated from the ratio of the amylase activity to the protein content. The research data were statistically tested using ANOVA and the multiple-distance Duncan method at a significance level of 5%. The results of the study showed that the highest amylase activity of 0.0084±0.0014 U/mL was produced in a medium with a nitrogen source of tempeh, the mineral was MgSO4.7H2O, a concentration of 0.05%. The protein content was 0.5111±0.0073 mg/ml and the specific activity was 0.0164±0.00 U/mg.

Downloads

Download data is not yet available.

References

Amin, A., Asnita., Nurul, I. B. & Hidayah. 2020. Pengaruh penambahan Ion Logam Natrium, Kalium, Magnesium, Kalsium pada biokonversi tepung jagung (Zea Mays L.) oleh Ragi Endomycopsis Fibuligera menjadi Senyawa Prebiotik. Fullerene Journ. Of Chem, 5(1): 32-39.
Bintang, M. 2010. Biokimia: Teknik penelitian. Penerbit Erlangga.
Boyer, R. 1993. Modern Experimental Biochemistry. Edisi 3. Benjamin Cummings, San Francisco.
Cahyadi, W. 2007. Kedelai. Jakarta: Bumi Aksara.
Dixon, M. and Webb, E.C. 1979. Enzymes. Academic Press, London.
Kizhakedathil, M. P. J., & C, S. D. 2021. Acid stable α-amylase from Pseudomonas balearica VITPS19—Production, purification, and characterization. Biotechnology Reports, 30, e00603.
Kumar, S. & Nussinov, R. 2001. How do Thermophilic Protein Deal with Heat. Cellular and Molecular Life Science, 58: 61-65.
Lévêque, E., Janeček, Š., Haye, B., & Belarbi, A. (2000). Thermophilic archaeal amylolytic enzymes. Enzyme and Microbial Technology, 26(1), 3–14.
Lorena. O. 2021. Produksi enzim amilase dari jamur termofilik Aspergillus sp. LBKURCC304 dengan variasi sumber karbon dalam media produksi cair. Skripsi. Riau, Universitas Riau.
Mahardikaningrum, S. dan Yuanita, L. 2012. Aktivitas enzim amilase Rattus norvegicus pada diet tinggi serat pangan : variasi ph dan lama perebusan. UNESA Journal of Chemistry. 1(1): 100-107.
Mohammad, B. T., Al Daghistani, H. I., Jaouani, A., Abdel-Latif, S., & Kennes, C. 2017. Isolation and Characterization of Thermophilic Bacteria from Jordanian Hot Springs: Bacillus licheniformis and Thermomonas hydrothermalis Isolates as Potential Producers of Thermostable Enzymes. International Journal of Microbiology, 2017.
Nofiani, R. 2012. Urgensi dan Mekanisme Biosintesis Metabolit Sekunder Mikroba Laut. Jurnal Natur Indonesia, 10(2), 120–125.
Saryono., Usman. P., & Ririn, N. 2018. Eksplorasi senyawa bioaktif termostabil dari mikroorganisme yang diisolasi dari sumber air panas di wilayahSumatra bagian tengah. Laporan penelitian PD-UPT tahun ke-1, LPPM Universitas Riau.
Sindhu, R., Binod, P., Madhavan, A., Beevi, U. S., Mathew, A. K., Abraham, A., Pandey, A., & Kumar, V. 2017. Molecular improvements in microbial α-amylases for enhanced stability and catalytic efficiency. Bioresource Technology, 245(Part B), 1740–1748.
Suhartono, M. T. 1978. Enzim dan bioteknologi. Alumni.
Tiwari, S.P., Srivastava, R., Singh, C.S., Shukla, K., Singh, R.K., Singh, R., Singh, N.L., Sharma, R. 2015. Amylases: an overview with specialreference to alpha-amylase. J. Glob. Biosci. 4(1): 1886–1901.
Unal, A. 2015. Production of α-amylase from some thermophilic Aspergillus species and optimization of its culture medium and enzyme activity. African Journal of Biotechnology. 14(47) : 3179-3183.
Wardani, R.L dan Rudiana, A. 2017. Effect of concentration yeast hydrolysate enzymatic (YHE) as supplements culture media for growth. Jurnal Kimia Unesa. 6(1).
Widylia. F. 2020. Analisis Produksi Enzim Amilase Dari Jamur Termofilik Aspergillus sp. LBKURCC304. Strain Lokal Bukik Gadang SumatraBarat. Skripsi. Riau: Universitas Riau
Published
2023-11-30
How to Cite
Mhd Muslim Syaifullah, Devi, S., Saryono, S., Itnawita, I., & Mukhlis, M. (2023). Optimization of Nitrogen and Mineral Sources in Liquid Media for Amylase Production Thermophilic Aspergillus sp. LBKURCC304. Photon: Journal of Natural Sciences and Technology, 14(1), 1-9. https://doi.org/10.37859/jp.v14i1.5468
Section
Chemical Sciences
Abstract views: 38 , PDF downloads: 24