Biodegradasi Hidrokarbon Crude Oil di Kawasan PT. Bumi Siak Pusako-Pertamina Hulu menggunakan Konsorsium Bakteri Indigen
Keywords:
Bakteri, biodegradasi, crude oil, konsorsium
Abstract
Riau Province is one of the largest producing areas of crude oil. Drilling and refining petroleum often produce waste crude oil in large quantities. One method that can be used to degrade waste is biodegradation using a consortium of microorganisms. This study examined the effectiveness of a consortium of indigenous bacteria in degrading hydrocarbon compounds. Growth tests and the ability of isolates to degrade hydrocarbons were carried out by inoculating isolates in liquid Bushnell Haas media containing crude oil of about 5% and incubating for 16 days. The potency of the indigen bacterial consortium during the degradation process is influenced by several parameters, including pH, OD (Optical Density), dan CO2 observed on days 0, 4, 8, 12 and 16 days of incubation. KB4 is the superior consortium in degrading hydrocarbon crude oil. Based on the analysis using GC-MS instruments, the bacteria consortium can degrade two hydrocarbon compounds in crude oil.
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References
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Yudono, B., Estuningsih, S. P., Said, M., & Napoleon, A. (2013). Eksplorasi Bakteria indigen Pendegradasi Limbah Minyak Bumi di Wilayah PT Pertamina UBEP Limau Muara Enim. Prosiding Semirata FMIPA Universitas Lampung, 127–134.
Antika, B., & Novianty, R. (2019). Penentuan kurva pertumbuhan pseudomonas sp. lbkurcc149. Repository University of Riau.
Darsa, K. V, Thatheyus, A. J., & Ramya, D. (2014). Biodegradation of Petroleum Compound Using the Bacterium Bacillus subtilis. Science International, 20–25. https://doi.org/10.17311/sciintl.2014.20.25
Fitrida, A., Apriyana, K., Astuti, R., & Novianty, R. (2019). Biodegradasi senyawa toksik naftalena pencemar lingkungan menggunakan isolat fungi indigen. Jurnal Ipteks Terapan, 13(2), 165–174.
Fitrida, A., Novianty, R., Saryono, Awaluddin, A., & Pratiwi, N. W. (2020). Optimasi pertumbuhan isolat fungsi indigen Penicilium sp. LBKURCC153 pendegradasi naftalena menggunakan glukosa sebagai kosubstrat pada minimal medium. Jurnal Inovasi Pendidikan Dan Sains, 01(01), 20–25.
Grifoni, M., Petruzzelli, G., Barbafieri, M., & Rosellini, I. (2017). Soil Quality Protection at Heavy Contaminated Manufactured Gas Plant Sites : Role of Biological Remediation. In Enchancing Cleanup of Environmental Pollutants (pp. 231–260). Springer International Publishing. https://doi.org/10.1007/978-3-319-55426-6
Katz, B., & Everett, M. A. (2016). An Overview of Pre-Devonian Petroleum Systems – Unique Characteristics and Elevated Risks. Marine and Petroleum Geology, 73(2), 116–126. https://doi.org/10.3997/2214-4609.20131811
Kermani, A. J. N., Ghasemi, M. F., Khosravan, A., Farahmand, A., & Shakibaie, M. R. (2013). Cadmium bioremediation by metal-resistant mutated bacteria isolated from active sludge of industrial effluent Cadmium bioremediation by metal resistant mutated bacteria isolated from active sludge. Iranian Journal of Environmental Health Science & Engineering, 7(4), 279–286.
Khan, M. A. I., Biswas, B., Smith, E., Naidu, R., & Megharaj, M. (2018). Toxicity assessment of fresh and weathered petroleum hydrocarbons in contaminated soil- a review. Chemosphere, 1–54. https://doi.org/10.1016/j.chemosphere.2018.08.094
Koshlaf, E., & Ball, A. S. (2017). Soil bioremediation approaches for petroleum hydrocarbon polluted environments. Microbiology, 3(1), 25–49. https://doi.org/10.3934/microbiol.2017.1.25
Lund, P. A., Biase, D. De, Nuno, P. M., Ceteciogfu, Z., Fernandez, E. N., Sara, B.-C., Hall, R., Sauer, M., & O’Byrne, C. (2020). Understanding How Microorganisms Respond to Acid pH Is Central to Their Control and Successful Exploitation. Frontiers in Microbiology, 11, 1–8. https://doi.org/10.3389/fmicb.2020.556140
Murali, N., Srinivas, K., & Ahring, B. K. (2017). Biochemical Production and Separation of Carboxylic Acids for Biorefinery Applications. Fermentantion, 3(22), 1–25. https://doi.org/10.3390/fermentation3020022
Novianty, R, Dahliaty, A., Nasution, N. I., & Haryati. (2020). Biodegradasi popok bayi bekas menggunakan jamut dan bakteri selulolitik dengan fermentasi padat. Jurnal Inovasi Pendidikan Dan Sains, 01(01), 13–19.
Novianty, R, Antika, B., & Awaluddin, A. (2022). Naphthalene degradation by Pseudomonas sp . LBKURCC419 strain with addition of glucose as cosubstrate. Biodiversitas, 23(11), 5654–5661. https://doi.org/10.13057/biodiv/d231115
Novianty, R, Antika, B., Saryono, Awaluddin, A., & Pratiwi, N. W. (2020). Potensi tiga isolat bakteri indigen dari Kabupaten Siak Provinsi Riau dalam Mendegradasi Naftalena. Jurnal Kimia, 14(41), 94–100.
Novianty, R, Awaluddin, A., & Pratiwi, N. W. (2020). Bakteri Indigen Pendegradasi Hidrokarbon Minyak Bumi di Kabupaten Siak Provinsi Riau. Jurnal Teknik Kimia USU, 09(1), 34–40.
Novianty, R, Saryono, Awaluddin, A., Pratiwi, N. W., Hidayah, A., & Juliantari, E. (2021). The diversity of fungi consortium isolated from polluted soil for degrading petroleum hydrocarbon. Biodiversitas, 22(11), 5077–5084. https://doi.org/10.13057/biodiv/d221145
Rosmawati, T., & Rijal, M. (2020). ISOLATION AND ACTIVITY TEST OF HYDROCARBON DEGRADING MOLD FROM THE PETROLEUM WASTE OF PT OLLOP BULA IN WEST SERAM DISTRICT. Pollution Research, 39, 1–8.
Sari, E. M., Novianty, R., Awaluddin, A., Saryono, & Pratiwi, N. W. (2019). Effectiveness of crude oil degrading fungi isolated from petroleum hydrocarbon contaminated soil in Siak, Riau. Acta Biochimica Indonesiana, 2(1), 15–22.
Sudrajat, D., Mulyana, N., & Retno, T. (2015). Isolasi dan aplikasi mikroba indigen pende- gradasi hidrokarbon dari tanah tercemar minyak bumi. Prosiding Pertemuan Dan Presentasi Ilmiah-Penelitian Dasar Ilmu Pengetahuan Dan Teknologi Nuklir, 101–109.
Vanishree, M., Thatheyus, A. J., & Ramya, D. (2014). Biodegradation of Petrol Using the Fungus Penicillium sp . Science International, 26–31. https://doi.org/10.17311/sciintl.2014.26.31
Wu, X., Gu, Y., Wu, X., Zhou, X., Zhou, H., Amanze, C., Shen, L., & Zeng, W. (2020). Construction of a Tetracycline Degrading Bacterial Consortium and Its Application Evaluation in Laboratory ‐ Scale Soil Remediation. Microorganisms, 8(292), 1–18. https://doi.org/10.3390/microorganisms8020292
Xu, X., Liu, W., Tian, S., Wang, W., Qi, Q., & Jiang, P. (2018). Petroleum Hydrocarbon-Degrading Bacteria for the Remediation of Oil Pollution Under Aerobic Conditions : A Perspective Analysis. Frontiers in Microbiology, 9, 1–11. https://doi.org/10.3389/fmicb.2018.02885
Yudono, B., Estuningsih, S. P., Said, M., & Napoleon, A. (2013). Eksplorasi Bakteria indigen Pendegradasi Limbah Minyak Bumi di Wilayah PT Pertamina UBEP Limau Muara Enim. Prosiding Semirata FMIPA Universitas Lampung, 127–134.
Published
2023-06-30
How to Cite
Novianty, R., & Yuharmen, Y. (2023). Biodegradasi Hidrokarbon Crude Oil di Kawasan PT. Bumi Siak Pusako-Pertamina Hulu menggunakan Konsorsium Bakteri Indigen. Photon: Jurnal Sain Dan Kesehatan, 13(2), 83-89. https://doi.org/10.37859/jp.v13i2.5053
Section
Chemistry Sciences
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