Carbera manghas Leaf Extract as Larvacide in Controlling Aedes aegypti

Abstract

Abstract. One method to control A.aegypti is by using synthetic larvacide (temephos);
however it can cause larval resistance. To impact and environmentally-friendly control
larvae, Carbera manghas leaf extract can be used. The study assesses the effectiveness
of the extract against A. aegypti larvae based on LC50 and LT50 to inhibit pupa growth.
20 instar larvae were used for each of 2500, 4000, 5500 and 7000 ppm concentrations
with positive/negative control, 6 treatments and 4 repetitions, observed every 6 hours
within 72 hours. One Way Anova and Probit Analysis were applied. The highest larval
mortality was in 7000 ppm, valued at 83.75%. With Kruskall Wallis test, P-value 0.000
< 0.05. Mann-Whitney test, it is suggested that the extract affects larval mortality and
inhibits the growth of pupae. The LC50 value is in 3971 ppm concentration within 72
hours. The LT50 values in 2500, 4000, 5500 and 7000 ppm concentrations are 10.8,
8.50, 7.10 and 6.11 hours respectively. 7000 ppm concentration is the highest in
inhibiting pupa growth, with 69.23%. The extract can be used as natural larvacide, yet
further researches on effects on human body and water purification treatments are
needed.