Acetone-Based Comparative Phytochemical Profiling of Indonesian Ethnomedicinal Plants: Effects of Maceration and Soxhlet Extraction
DOI:
https://doi.org/10.37859/jst.v13i1.11209
Abstract
Acetone has rarely been applied as a single solvent in comparative phytochemical studies of Indonesian medicinal plants, and systematic comparisons of extraction techniques remain limited. This study aimed to evaluate the qualitative phytochemical profiles and potential bioactivity of acetone extracts obtained using maceration and Soxhlet extraction from selected ethnomedicinal plants. The samples included the leaves and fruits of Pereskia bleo, leaves and stems of Gnetum gnemon, leaves of Bouea macrophylla, leaves of Psidium guajava, and the banana inflorescence of Musa paradisiaca. Phytochemical screening revealed that phenolics, steroids, and terpenoids were consistently detected in all samples. Strong flavonoid presence (+++) was observed in several plant parts, particularly P. bleo fruit and G. gnemon. Alkaloids and glycosides were detected only in maceration extracts, reflecting their thermolabile nature. These results demonstrate that acetone effectively profiles secondary metabolites, while extraction methods significantly influence the detection of thermosensitive compounds.
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References
H. J. Woerdenbag and O. Kayser, "Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use," J. Herb. Med., vol. 4, no. 2, pp. 51–73, 2014.
N. N. Azwanida, "A review on the extraction methods used in medicinal plants: principle, strength and limitation," Med. Aromat. Plants, vol. 4, no. 196, 2015.
M. D. L. De Castro and F. Priego-Capote, "Soxhlet extraction: Past and present panacea," J. Chromatogr. A, vol. 1217, no. 16, pp. 2383–2389, 2010.
S. Sun et al., "Impact of extraction techniques on phytochemical composition and bioactivity of natural product mixtures," Front. Pharmacol., vol. 16, art. 1615338, 2025.
L. G. M. da Silva et al., "Synergy between solvent polarity and composition for efficient extraction of bioactive compounds from pitaya," Food Mater. Res., vol. 5, no. 1, 2025.
M. Naczk and F. Shahidi, "Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis," J. Pharm. Biomed. Anal., vol. 41, no. 5, pp. 1523–1542, 2006.
C. Reichardt and T. Welton, Solvents and Solvent Effects in Organic Chemistry. Hoboken, NJ, USA: John Wiley & Sons, 2011.
A. J. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Dordrecht, Netherlands: Springer Science & Business Media, 1998.
W. C. Evans, Trease and Evans Pharmacognosy, 15th ed. India: Saunders/Elsevier Science, 2002.
A. E. Sofowara, Medicinal Plants and Traditional Medicine in Africa, vol. 2. Ibadan, Nigeria: Spectrum Books Ltd., 1993.
K. S. Sim, A. M. Sri Nurestri, and A. W. Norhanom, "Phenolic content and antioxidant activity of crude and fractionated extracts of Pereskia bleo (Kunth) DC. (Cactaceae)," African J. Pharm. Pharmacol., vol. 4, no. 5, pp. 193–201, 2010.
S. Zareisedehizadeh, C.-H. Tan, and H.-L. Koh, "A review of botanical characteristics, traditional usage, chemical components, pharmacological activities, and safety of Pereskia bleo (Kunth) DC.," Evidence-Based Complement. Altern. Med., vol. 2014, art. 326107, 2014.
R. M. P. Gutierrez, S. Mitchell, and R. V. Solis, "Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology," J. Ethnopharmacol., vol. 117, no. 1, pp. 1–27, 2008.
Z. Khan, D. Shahwar, M. K. Y. Ansari, and R. Chandel, "Toxicity assessment of anatase (TiO2) nanoparticles: A pilot study on stress response alterations and DNA damage studies in Lens culinaris Medik.," Heliyon, 2019.
I. T. Fu’adah, S. A. Sumiwi, and G. Wilar, "The evolution of pharmacological activities of Bouea macrophylla Griffith in vivo and in vitro study: A review," Pharmaceuticals, vol. 15, no. 2, art. 238, 2022.
N. T. Huong, N. Q. Hop, D. A. Duy, and N. T. Son, "The genus Gnetum: Traditional use, phytochemistry, nutritional value, biosynthesis, synthesis, pharmacology, toxicology, synthetic advance, and pharmacokinetics," Fitoterapia.
C. Riviere, A. D. Pawlus, and J.-M. Merillon, "Natural stilbenoids: Distribution in the plant kingdom and chemotaxonomic interest in Vitaceae," Nat. Prod. Rep., vol. 29, no. 11, pp. 1317–1333, 2012.
H. Kato, M. Samizo, R. Kawabata, F. Takano, and T. Ohta, "Stilbenoids from the melinjo (Gnetum gnemon L.) fruit modulate cytokine production in murine Peyer’s patch cells ex vivo," Planta Med., vol. 77, no. 10, pp. 1027–1034, 2011.
F. A. A. F. Gadelha et al., "Musa paradisiaca L. inflorescence abrogates neutrophil activation by downregulating TLR4/NF-κB signaling pathway," 2021.
S. Naseer, S. Hussain, N. Naeem, M. Pervaiz, and M. Rahman, "The phytochemistry and medicinal value of Psidium guajava (guava)," Clin. Phytosci., vol. 4, no. 1, art. 32, 2018.
G. Rajkumar, M. R. Jayasinghe, and S. Vinotha, "Comparative analytical study of phytochemicals in selected antidiabetic medicinal plant seeds in Sri Lanka," 2021.
A. Sangkal, R. Ismail, and N. S. Marasabessy, "Identifikasi senyawa metabolit sekunder ekstrak daun bintaro (Cerbera manghas L.) dengan pelarut etanol 70%, aseton dan n-heksan," J. Sains dan Kesehat., vol. 4, no. 1, 2020.
A. R. Shabira, L. H. Fadiah, and M. Umami, "Analisis senyawa fitokimia dari ekstrak maggot black soldier fly (Hermetia illucens)," Zoologi: J. Ilmu Peternakan, Ilmu Perikanan, Ilmu Kedokteran Hewan, vol. 3, no. 2, pp. 51–63, 2025.
B. Kalita, M. Paul, N. Devi, and B. Tanti, "A comparative study on proximate and phytochemical analysis of Clausena excavata Burm. f. and C. heptaphylla (Roxb.) Wight & Arn occurring in Assam (India)," Vegetos.
I. Ignat, I. Volf, and V. I. Popa, "A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables," Food Chem., vol. 126, no. 4, pp. 1821–1835, 2011.
S. F. Sulaiman, A. A. B. Sajak, K. L. Ooi, and E. M. Seow, "Effect of solvents in extracting polyphenols and antioxidants of selected raw vegetables," J. Food Compos. Anal., vol. 24, no. 4–5, pp. 506–515, 2011.
S.-C. Liu, J.-T. Lin, C.-K. Wang, H.-Y. Chen, and D.-J. Yang, "Antioxidant properties of various solvent extracts from lychee (Litchi chinensis Sonn.) flowers," Food Chem., vol. 114, no. 2, pp. 577–581, 2009.
M. A. R. Mazumder, A. Tolaema, P. Chaikhemarat, and S. Rawdkuen, "Antioxidant and anti-cytotoxicity effect of phenolic extracts from Psidium guajava Linn. leaves by novel assisted extraction techniques," Foods, 2020.
M. A. Johari and H. Y. Khong, "Total phenolic content and antioxidant and antibacterial activities of Pereskia bleo," 2019.
M. M. Cowan, "Plant products as antimicrobial agents," Clin. Microbiol. Rev., vol. 12, no. 4, pp. 564–582, 1999.
B. S. Padam, H. S. Tin, F. Y. Chye, and M. I. Abdullah, "Extracts of banana (Musa paradisiaca cv. Mysore) inflorescences," J. Biol. Sci., vol. 12, no. 2, pp. 62–73, 2012.
S. F. Mohd-Salleh, N. Ismail, W. S. Wan-Ibrahim, and T. N. N. Tuan Ismail, "Phytochemical screening and cytotoxic effects of crude extracts of Pereskia bleo leaves," J. Herbs Spices Med. Plants, vol. 26, no. 3, pp. 291–302, 2020.
A. Sukohar and D. A. Ramdini, "Investigation of antioxidant and anticancer activity against MCF-7 and HeLa cancer cells of melinjo (Gnetum gnemon L.)," , vol. 16, pp. 2177–2185, 2023.
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Copyright (c) 2026 Andi Malik Hakim, Rina Mulya Lastriana, Putri Dwinta Nurmalasari, Rahma Tiara Alfatiha, Firman Wahyu R, Maesaroh Maesaroh, Sumaah Sumaah, Nurul Oktaviani Handia Putri, Nauromunnisa Nauromunnisa, Muhammad Islam Abdurrazaq Shiddiq, Restu Indrianti Wulandari
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