Uji Aktivitas Sitotoksik Ekstrak Kulit Batang Tumbuhan Akar Kaik-Kaik (Uncaria Cordata) (Lour.) Meer Dengan Metode Brine Shrimp Lethality Test (BSLT)
Abstract
In this study, a cytotoxic test of the root bark extract of kaik-kaik (Uncaria cordata)(Lour.)Merr was carried out using the Brine Shrimp Lethality Test (BSLT) method. The root bark extract of kaik-kaik (Uncaria cordata) (Lour.) Merr was obtained by maceration using n-hexane, ethyl acetate, and ethanol as solvents. The effect of each extract was identified on the percentage of mortality of Artemia salina Leach larvae and a probit analysis was calculated. The results showed that the three extracts provided cytotoxic activity with active categories with LC50 respectively, namely, 45.28 ug / ml, 55.08 ug / ml, 47.09 ug / ml which could be used as candidates for anti-cancer drugs
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References
Bacher, N., Tiefenthaler, M., Sturm, S., Stuppner, H., Ausserlechner, M. J., Kofler, R., dan Konwalinka, G., 2005. Oxindole alkaloids from Uncaria tomentosa Induce Apoptosis in Proliferating, G0/G1Arrested and bcl-2-Expressing Acute Lymphoblastic Leukemia Cells, British Journal of Hematology, 123: 615622
Departemen Kesehatan Republik Indonesia. 2007. Pencegahan Kanker Leher Rahim dan Kanker Payudara. Jakarta
Djamal, R. (1998). Prinsip-prinsip dasar kerja dalam kimia bahan alam. Padang: FMIPA Universitas Andalas.
Fox, R. 2004. Statistic Acute Toxicity Bioassay Laboratory Exercise. Laboratory Ecology: 306.
Meyer, B. N., Feerigni; N. R., Putnam, J. E., Jacobson, L, B., Nicholas, D, E. dan McLaughlin, J, L. 1982. Brine shrimp: a convenient general bioassay for active plant constituens. Plant Medical. 45: 31-34.
Nussbaumer, S; Bonnabry, P; Veuthey, JL; dan Sandrine, F. 2011. Analysis of Anticancer Drugs. A review. 85: 2265-2289
Setyowati, W.A.E, dkk. (2014). Skrining Fitokimia dan Identifikasi Komponen Utama Ekstrak Metanol Kulit Durian (Durio zibethinus Murr.) Varietas Petruk. Jurnal Seminar Nasional Kimia dan Pendidikan Kimia VI. ISBN ( 979363175-0): 271-280.