Detection of Gene E6 HPV 18 With Real Time PCR (Polymerase Chain Reaction) Using Isolated Samples of Uterine Cancer Patients in Riau Province
Keywords:
Gene E6, HPV 18, cervical cancer, RT PCR
Abstract
One indication that there has been malignancy in cervical lesions due to exposure to high-risk hpv types such as HPV 16 and 18 is the detection of oncogene DNA in the cervical tissue of cancer patients. The oncogenes are the E6 and E7 genes. Previous research data showed evidence that the prognosis of HPV 18 is more massive than that of other high-risk HPV types such as HPV 16. Thus, the effort to detect the E6 gene from biopsy samples of patients suspected of cervical cancer is the right and effective step in order to establish an accurate diagnosis of cervical cancer itself, especially caused by HPV 18.
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References
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Boulet, G., Horvath, C., Broeck, D. V., Sahebali, S., & Bogers, J. (2007). Human papillomavirus: E6 and E7 oncogenes. The international journal of biochemistry & cell biology, 39(11), 2006-2011.
Branislav Ruttkay-Nedecky, Anna Maria Jimenez Jimenez, Lukas Nejdi, Dagmar Chudova, Jaromir Gumulec, Michal Masarik, Vojtech Adam and Rene Kizek, Relevance of infection with human papillomavirus : The role of the p53 tumor suppressor protein and E6/E7 zinc finger proteins (Review), International Journal of oncology, Vol 43, 2013
Brian J. Morris, Cervical human papillomavirus by PCR : advantages of targetting the E6/E7 region, Clin Chem Lab Med 2005
De Boer, M. A., Peters, L. A., Aziz, M. F., Siregar, B., Cornain, S., Vrede, M. A.,& Fleuren, G. J. (2005). Human papillomavirus type 18 variants: histopathology and E6/E7 polymorphisms in three countries. International journal of cancer, 114(3), 422-425.
Ethel-Michele de Villiers, Claude Fauquet, Thomas R. Broker, Hans Ulrich Bernard and Harold zur Hausen, Classification of papillomaviruses, Virology 324 (2004) 17-27
Heather L. Howie, Rachel A. Katzenellenbogen, Denise A. Galloway, Papillomavirus E6 Proteins, Virology 384, 2009
IARC Monographs Volume 90
JNI Vet, MA de Boer, BE, BEWM van deb Akker, B Siregar, Lisnawati, S Budiningsih, D Tyasmorowati, Moestikaningsih, S Cornain, AAW Poters and GJ Fleuren, Prevalence of human papillomavirus in Indonesia : a population-based study in three regions, British Journal of Cancer (2008) 99, 214-218
Maaike C. Schellekens, Anneke Dijkman, Mohammad Farid Aziz, Budiningsih Siregar, Santoso Cornain, Sandra Kolkman-Uljee, Lex A. W. Peters and Gert Jan Fleuren, Prevalence of single and multiple HPV types in cervical carcinomas in Jakarta, Indonesia, Gynecologic Oncology 93 (2004) 49-53
Mark S. Lechnert and Laimonis A. Laimins, Inhibition of p53 DNA Binding by Human Papillomavirus, International Journal of Virology, July 1994
Basic Health Research 2013, Results of Health Research and Development, Ministry of Health of the Republic of Indonesia, 2013
Villa LL, Sichero L, Rahal P, Caballero O, Ferenczy A, et al. (2000) Molecular variants of human papillomavirus types 16 and 18 preferentially associated with cervical neoplasia. J Gen Virol 81: 2959–2968.
WHO, Evidence based recommendations on Human Papilloma Virus (HPV), Vaccines Schedules , Background paper for SAGE discussions, March 11, 2014
WHO/ICO, 2015
Wynn H. Kao, Sylviel, Beaudenon, Andrea L. Talis, Jon M. Huibregtse and Peter M. Howley, Human Papillomavirus Type 16 E6 Induces Self-Ubiquitination of the E6AP Ubiquitin-Protein Ligase, Journal of Virology, July 2000
Published
2022-02-22
How to Cite
Hari, D. G. (2022). Detection of Gene E6 HPV 18 With Real Time PCR (Polymerase Chain Reaction) Using Isolated Samples of Uterine Cancer Patients in Riau Province. As-Shiha: Jurnal Kesehatan, 1(2), 88-93. Retrieved from https://ejurnal.umri.ac.id/index.php/JKU/article/view/3354
Section
Articles
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