PELATIHAN PROSEDUR LABORATORIUM ANALISIS DNA

  • Dewi Indriyani Roslim Universitas Riau
  • Herman Herman
  • Roza Elvyra
  • Nery Sofiyanti
  • Ennie Chahyadi
Keywords: DNA isolation, Electrophoresis, laboratory procedure, PCR, workshop

Abstract

Currently, analysis based on DNA is one of the techniques for solving the problems such as paternity test, determining the identity of accident victims, natural disasters victims, and authentication of foods ingredients. But not everyone knows about the stages of DNA analysis laboratory procedures. The purpose of this workshop is to provide knowledgments about DNA analysis laboratory procedures. The training was attended by 30 participants from various universities and research centers in Riau Province. Workshop materials include DNA isolation of animal, plant and bacterial, electrophoresis, polymerase chain reaction (PCR), and visualization of DNA bands. Evaluation uses a questionnaire distributed to participants. Questionnaire analysis showed that workshop was very useful especially for beginners who want to be involved in DNA analysis activities in the laboratory.

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References

[1] Balai Besar POM. 2010. Peningkatan kapasitas laboratorium dengan alat PCR (polymerase chain reaction) untuk uji kehalalan produk. Mataram: BPOM. [diakses 29 Maret 2015 http://www.pom.go.id/new/index.php/view/ berita/495/].
[2] Fahmi K. 2013. BPOM: Produk Nestle di Indonesia Steril DNA Kuda. [diakses 29 Maret 2015 http://www.lensaindonesia.com/2013/02/23/bpom-produk-nestle-diindonesia-steril-dna-kuda.html].
[3] Gill P, Jeffreys AJ, Werrett DJ. 1985. Forensic application of DNA fingerprints. Nature 318: 577–579.
[4] Jeffreys AJ, Allen MJ, Hagelberg E, Sonnberg A. 1992. Identiǔcation of the skeletal remains of Josef Mengele by DNA analysis. Forensic Sci Int 56: 65–76.
[5] Narayanan NN, Ihemere U, Ellery C, Sayre RT. 2011. Overexpression of hydroxynitrile lyase in cassava roots elevates protein and free amino acids while reducing residual cyanogen levels. PLoS ONE 6(7): e21996. doi:10.1371/journal.pone.0021996.
[6] Roslim DI, Herman, Elvyra R, Sofiyanti N, Chahyadi E. 2017. IbIKK Klinik Analisis DNA. Prosiding Konferensi Nasional PKM-CSR ke-3 tahun 2017. Surakarta, 19-21 Oktober 2017.
[7] Saad R. 2005. Discovery, development, and current application of DNA identitiy testing. BUMC Proceedings 18:130-133.
Published
2018-11-23
How to Cite
Roslim, D. I., Herman, H., Elvyra, R., Sofiyanti, N., & Chahyadi, E. (2018). PELATIHAN PROSEDUR LABORATORIUM ANALISIS DNA. Jurnal Pengabdian UntukMu NegeRI, 2(2), 44-48. https://doi.org/10.37859/jpumri.v2i2.873
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