Prebiotic Test of Three Variety of Mushrooms (Auricularia polytricha, Agaricus bisporus, and Peluretus cystidiosus) Towards “Lactobacillus casei” Bacteria

  • Hasmalina Nasution Dept. of Chemistry, Faculty of Mathematics and Natural Sciences and Health, Muhammadiyah University of Riau.
  • Rahayu Rahayu Dept. of Chemistry, Faculty of Mathematics and Natural Sciences and Health, Muhammadiyah University of Riau.
  • Musyirna Rahmah Nasution Pharmaceutical Sciences Study Program, College of Pharmaceutical Sciences Riau.

Abstract

One formulation of maintenance of normal intestinal microbiota for diarrhea
therapy in infants and children can use mushroom prebiotics. Mushroom
polysaccharides are a potential source of prebiotics because they contain nutrients such
as chitin, hemicellulose, α & -glucans, mannas, xylans and galactose. Prebiotics
cannot be separated from probiotics because the prebiotic target stimulates selective
growth of probiotic bacteria. One of the probiotic bacteria is Lactobacillus casei. The
purpose of this study is to determine the prebiotic activity of several fungi against
Lactobacillus casei. The mushrooms used are black ear mushroom (Auricularia
polytricha), white button mushroom (Agaricus bisporus) and brown oyster mushroom
(Pleurotus cystidiosus) with 4 variations of concentration (3.125% b / v, 6.25% b / v,
12.5% b / v and 25% b / v) affecting the growth of Lactobacillus casei bacteria measured
by absorbance of bacteria using a spectrophotometer at a wavelength of 434 nm. Black
ear mushroom (Auricularia Polytricha), white button mushroom (Agaricus bisporus)
and brown oyster mushroom (Pleurotus cystidiosus) have the potential to be a prebiotic
to the Lactobacillus casei bacteria. The highest prebiotic activity is brown oyster
mushroom (Pleurotus cystidiosus). Result analysis in this research is using Two-Way
Anova.

Published
2018-10-09
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