Phytochemical Screening and KLT Analysis of Endophytic Fungi Secondary Metabolite of Vetiveria zizanioides L.

Abstract

Abstract. Endophytic fungi are microorganisms that live in the healthy tissue of the
host plant without causing disease. Endophytic fungi grow in every plant, including
vetiver roots of Vetiveria zizanioides L. both intracellular and intercellular. This study
aims to do phytochemical screening and Thin Layer Chromatography(KLT) analysis
of secondary metabolites produced by endophytic fungi from vetiver roots. The results
showed that secondary metabolites produced by endophytic fungi contain one or more
compounds, namely in the form of terpenoids, steroids, alkaloids, phenolics,
flavonoids, and saponins. All fungal isolates produce secondary metabolites in the form
of alkaloids except IH2 and IH11 isolates. The results ofKLT strengthened the
phytochemical screening results on secondary metabolites produced by endophytic
fungi with Rf values of 0.6-0.8.