Determination of Total Phenolic, Flavonoid Content Andfree Radical Scavenging Activity of Etanol Extract Sawo Stem Bark (Manilkara Zapota (L.))

  • Rahmiwati Hilma Chemistry Dept of Universitas Muhammadiyah Riau
  • Herliani Herliani Chemistry Dept of Riau University
  • M. Almurdani Chemistry Dept of Riau University


Abstract. Sawo (Manilkarazapota (L.) van Royen) was a plant used
traditionally by the community to treat various diseases. The purpose of this
study was to determine the content of total phenols, flavonoids and the activity
of free radical scavenging ethanol extract of the stem bark of M. zapota (L.) van
Royen. The stem bark extraction sample was carried out by maceration method
using ethanol. Test of total phenol and flavonoids content using the Folin-
Ciocalteu method, free radical scavenging activity using DPPH (2,2-diphenyl-
1-pikril-hidrazil) method. From the phytochemical test results showed that
positive extracts contain phenolic and flavonoid. The results showed that the
yield of stem bark ethanol extract was 25.11% with a total phenolic content was
12.97 mgGAE/g extract. Total flavonoid levels was 8.75 mgQE/g extract. The
activity of free radical scavenging extract (IC50) values of 19.69 ppm.


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